The restorations with dental alloys have toxic as well as mechanical stimulation to gingival tissue when they were contacted for long time.
Cell culture method is one of the tests for evaluation of biocompatibility of the material or its components. In this study, the cytotoxicity of type III dental crown and bridge casting alloys, dental amalgams, dental amalgam alloy containing
metals,
and copper-alluminum alloys was evaluated.
L 929 cells were cultured in Eagle's MEM supplemented with 10% fetal bovine serum, 100 IU/ml of penicillin, and 100 μg/ml of streptomycin and seeded with 5X10E5 cells/ml in plastic dishes (100×15 mm). After the cell monolayer had formed, medium
was
removed and the medium containing agarose was added. Thecells were stained with 0.01% neutral red solution and the specimens wee placed on the agar. After incubation for 24 hours at 37℃ in the CO2 incubator, the cultures were examined
macroscopically
and microscopically under a microscope to determine the cytotoxicity.
@ES From the experiments, the following results were obtained:
@EN 1. Type III dental crown and bridge casting alloys, dental ceramic alloys, dental Co-Cr alloys, pure silver, and pure tin showed mild cytotoxicity with zone index of 1.0-1.5 and lysis index of 1.0-2.0.
2. Copper-based alloy (NPG), after ethylene oxide gas sterilization, showed mild cytotoxicity with response index of 1.0/2.0; after autoclave sterilization, showed moderate moderate cytotoxicity with response index of 2.0/3.0.
3. Dental high copper amalgam (HVA) showed moderated cytotoxicity with response index of 2.0/3.0.
4. Of the constituent metals of dental amalgam alloy, copper showed severe cytotoxicity with response index of 4.0/4.5, and zinc showed moderate cytotoxicity with response index of 3.0/3.3.
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